The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 28, 2021

Filed:

May. 08, 2017
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Daniel Irimia, Charlestown, MA (US);

Felix Ellett, Jamaica Plain, MA (US);

Julianne Jorgensen, Somerville, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 15/14 (2006.01); G01N 15/02 (2006.01); G01N 15/10 (2006.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502753 (2013.01); G01N 15/0205 (2013.01); G01N 15/1031 (2013.01); G01N 15/1056 (2013.01); G01N 15/1459 (2013.01); G01N 15/1463 (2013.01); G01N 15/1484 (2013.01); G01N 33/487 (2013.01); G01N 33/5091 (2013.01); G01N 33/54366 (2013.01); B01L 2200/0694 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/086 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1075 (2013.01); G01N 2015/1488 (2013.01); G01N 2015/1493 (2013.01); G01N 2800/26 (2013.01);
Abstract

The disclosure relates to microfluidic devices and methods of use thereof for monitoring the directionality, velocity, and migration persistence of neutrophils or other cells in the absence of chemical gradients for the purposes of detecting and quantifying abnormal neutrophil motility phenotypes, using low sample volumes and with minimal activation of the neutrophils. The devices and methods can be used to diagnose sepsis in subjects suspected of having sepsis or at risk of developing sepsis. The devices and methods can also be used to monitor the responses of patients to sepsis therapies.


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