The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 21, 2021
Filed:
Oct. 16, 2015
Applicant:
Carnegie Mellon University, Pittsburgh, PA (US);
Inventors:
Bruce Alan Armitage, Pittsburgh, PA (US);
Veronica Hinman, Pittsburgh, PA (US);
Munira F. Fouz, Pittsburgh, PA (US);
Assignee:
Carnegie Mellon University, Pittsburgh, PA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6841 (2018.01); C12Q 1/682 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/6818 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6818 (2013.01);
Abstract
Provided herein are methods of detecting target analytes, such as nucleic acids, for example microRNAs using an enhanced Tyramide Signal Amplification (TSA) method that employs probes tagged with tyramide-binding groups to amplify the effects of the TSA. The accessibility of the tyramide-binding groups, such as hydroxyphenyl groups, provides for large improvements in signal due to faster reaction with the radicals. The present invention further includes the application of the assay for detecting specific microRNAs.