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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11124806 B1

PDFFull Text
Date of Patent:
Sep. 21, 2021

Filed:

Nov. 17, 2016

Methods for producing isobutene from 3-methylcrotonic acid

Applicants:

Global Bioenergies, Evry, FR;

Scientist of Fortune, S.a., Luxembourg, LU;

Inventors:

Mathieu Allard, Saint-Vrain, FR;

Maria Anissimova, Nozay, FR;

Philippe Marlière, Tournai, BE;

Assignees:

Global Bioenergies, Evry, FR;

Scientist of Fortune, SA, Luxembourg, LU;

Attorneys:

Michele M. Wales

InHouse Patent Counsel, LLC

Primary Examiner:

Maryam Monshipouri

Int. Cl.
CPC ...
C12P 5/02 (2006.01); C12N 9/00 (2006.01); C12N 15/52 (2006.01); C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12P 7/40 (2006.01); C12N 9/16 (2006.01);
U.S. Cl.
CPC ...
C12P 5/026 (2013.01); C12N 9/1029 (2013.01); C12N 9/1085 (2013.01); C12N 9/1217 (2013.01); C12N 9/13 (2013.01); C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12N 15/52 (2013.01); C12P 7/40 (2013.01); C12Y 205/01 (2013.01); C12Y 207/02007 (2013.01); C12Y 207/02014 (2013.01); C12Y 207/02015 (2013.01); C12Y 208/03001 (2013.01); C12Y 208/03008 (2013.01); C12Y 301/02001 (2013.01); C12Y 301/0202 (2013.01); C12Y 301/02018 (2013.01); C12Y 401/01006 (2013.01); C12Y 401/01063 (2013.01); C12Y 604/01004 (2013.01); C12Y 604/01005 (2013.01);
Abstract

Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-methylcrotonyl-CoA into 3-methylcrotonic acid or wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-hydroxyisovalerate (HIV) into 3-methylcrotonic acid. It is described that the enzymatic conversion of 3-methylcrotonic acid into isobutene can, e.g., be achieved by making use of a 3-methylcrotonic acid decarboxylase, preferably an FMN-dependent decarboxylase associated with an FMN prenyl transferase, an aconitate decarboxylase (EC 4.1.1.6), a methylcrotonyl-CoA carboxylase (EC 6.4.1.4), or a geranoyl-CoA carboxylase (EC 6.4.1.5).

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