The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 21, 2021
Filed:
May. 12, 2019
Tianjin University of Science and Technology, Tianjin, CN;
Xixian Xie, Tianjin, CN;
Ning Chen, Tianjin, CN;
Heyun Wu, Tianjin, CN;
Guoliang Li, Tianjin, CN;
Qiang Li, Tianjin, CN;
Xiaoguang Fan, Tianjin, CN;
Qingyang Xu, Tianjin, CN;
Chenglin Zhang, Tianjin, CN;
Yanjun Li, Tianjin, CN;
Qian Ma, Tianjin, CN;
Tianjin University of Science and Technology, Tianjin, CN;
Abstract
The present disclosure relates to a genetically engineered strain with high production of uridine and its construction method and application. The strain was constructed as follows: heterologously expressing pyrimidine nucleoside operon sequence pyrBCAKDFE (SEQ ID NO:1) on the genome ofprompted by strong promoter Pto reconstruct the pathway of uridine synthesis; overexpressing the autologous prsA gene coding PRPP synthase by integration of another copy of prsA gene promoted by strong promoter Pon the genome; deficiency of uridine kinase, uridine phosphorylase, ribonucleoside hydrolase, homoserine dehydrogenase I and ornithine carbamoyltransferase. When the bacteria was used for producing uridine, 40-67 g/L uridine could be obtained in a 5 L fermentor after fermentation for 40-70 h using the technical scheme provided by the disclosure with the maximum productivity of 0.15-0.25 g uridine/g glucose and 1.5 g/L/h respectively which is the highest level of fermentative producing uridine reported at present.