The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 21, 2021
Filed:
Oct. 06, 2011
Tatsumi Yamazaki, Tokyo, JP;
Hisafumi Okabe, Shizuoka, JP;
Shinta Kobayashi, Helios, SG;
Yu Jau Chen, Helios, SG;
Atsuhiko Kato, Shizuoka, JP;
Masami Suzuki, Shizuoka, JP;
Koichi Matsubara, Helios, SG;
Tatsumi Yamazaki, Tokyo, JP;
Hisafumi Okabe, Shizuoka, JP;
Shinta Kobayashi, Helios, SG;
Yu Jau Chen, Helios, SG;
Atsuhiko Kato, Shizuoka, JP;
Masami Suzuki, Shizuoka, JP;
Koichi Matsubara, Helios, SG;
CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo, JP;
Abstract
The purpose of the present invention is to provide: a cancer stem cell mass from which cells incapable of forming cancer are substantially removed and which has a characteristic property of reproducing a layered structure of a cancer tissue; a process for producing the cancer stem cell mass; and use of the cancer stem cell mass. For achieving the purpose, the present inventors grew a human cancer tissue repeatedly in a NOG mouse, separated cancer cells from the grown cancer tissue, and made a comparison of various cancer cell culture processes with each other. As a result, a cancer stem cell composition which is homogeneous and is substantially free of the coexistence of cells capable of forming cancer and cells incapable of forming cancer in a mixed state can be produced successively by employing an attached culture process using a serum-free stem cell culture medium rather than a generally employed floating culture process, and consequently the present invention has been accomplished.