logo

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11118155 B1

PDFFull Text
Date of Patent:
Sep. 14, 2021

Filed:

Apr. 05, 2016

Intercellular separation method for cultured cells

Applicant:

Medikan Inc., Seoul, KR;

Inventor:

Hee Young Lee, Seoul, KR;

Assignee:

MEDIKAN INC., Seoul, KR;

Attorneys:

Weiss & Moy, P.C.

Jeffrey D. Moy

Primary Examiner:

Lucas A Stelling

Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/42 (2006.01); C12N 1/02 (2006.01); C12N 5/00 (2006.01); C12Q 1/24 (2006.01); G01N 1/28 (2006.01); G01N 1/40 (2006.01); G01N 33/18 (2006.01);
U.S. Cl.
CPC ...
C12N 1/02 (2013.01); C12M 1/12 (2013.01); C12M 29/04 (2013.01); C12M 33/04 (2013.01); C12M 33/12 (2013.01); C12M 33/14 (2013.01); C12M 47/02 (2013.01); C12M 47/04 (2013.01); C12M 47/08 (2013.01); C12N 5/00 (2013.01); C12Q 1/24 (2013.01); G01N 1/286 (2013.01); C12M 1/123 (2013.01); C12M 35/04 (2013.01); C12M 47/06 (2013.01); C12N 2509/10 (2013.01); G01N 33/1866 (2013.01); G01N 2001/4088 (2013.01);
Abstract

The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 μm to 300 μm; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

Find Patent Forward Citations

Loading…