The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 07, 2021
Filed:
Feb. 16, 2017
Applicants:
The University of Tokyo, Tokyo, JP;
Centre National DE LA Recherche Scientifique (Cnrs), Paris, FR;
Inventors:
Yannick Rondelez, Tokyo, JP;
Guillaume Gines, Tokyo, JP;
Kevin Montagne, Tokyo, JP;
Teruo Fujii, Tokyo, JP;
Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6844 (2013.01); C12Q 1/6876 (2013.01);
Abstract
An object is to provide a method of eliminating background amplification in detection of rare nucleic acids based on isothermal amplification. The method comprising steps of: preparing a mixture including solution and enzymes, and adding a first and a second template into the mixture, wherein the first template is an amplification template, and the second template is a leak absorption template. Methods to use this approach for multiplexed, ultrasensitive and ultra-specific detection of nucleic acids are presented.