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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11111517 B1

PDFFull Text
Date of Patent:
Sep. 07, 2021

Filed:

Apr. 21, 2017

Hba1c dehydrogenase

Applicant:

Kikkoman Corporation, Noda, JP;

Inventors:

Atsushi Ichiyanagi, Chiba, JP;

Yosuke Masakari, Chiba, JP;

Assignee:

Kikkoman Corporation, Chiba, JP;

Attorney:

Foley & Lardner LLP

Primary Examiner:

Jennifer Wecker

Assistant Examiner:

Michael Paul Shimek

Int. Cl.
CPC ...
C12Q 1/32 (2006.01); C12N 15/09 (2006.01); C12M 1/40 (2006.01); G01N 33/483 (2006.01); G01N 27/416 (2006.01); G01N 33/49 (2006.01); G01N 27/327 (2006.01); C12N 9/88 (2006.01); C12Q 1/00 (2006.01); G01N 33/72 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/006 (2013.01); C12M 1/40 (2013.01); C12N 9/0036 (2013.01); C12N 15/09 (2013.01); C12Q 1/32 (2013.01); G01N 27/327 (2013.01); G01N 27/3271 (2013.01); G01N 27/416 (2013.01); G01N 33/483 (2013.01); G01N 33/49 (2013.01); G01N 33/723 (2013.01); C12N 9/88 (2013.01);
Abstract

This invention provides an HbA1c dehydrogenase that is capable of directly acting on hemoglobin A1c and is less likely to be influenced by oxygen concentration and a method for measurement and a kit of assay reagents using such HbA1c dehydrogenase. The HbA1c dehydrogenase having dehydrogenase activity and capable of directly acting on HbA1c is obtained by substitution of one or more amino acid residues at positions corresponding to positions 280, 269, 54, 241, and 267 of the amadoriase that is capable of directly acting on hemoglobin A1c and is derived from, for example, the genus. This invention also provides a method for measurement of HbA1c, a kit of assay reagents, and a sensor using such HbA1c dehydrogenase. Such HbA1c dehydrogenase is capable of directly acting on hemoglobin A1c and has lowered oxidase activity and/or enhanced dehydrogenase activity. This not only eliminates the need for treatment of hemoglobin A1c with a protease but also enables the use of an electron mediator in the measurement of HbA1c, thereby reducing effects due to oxygen concentration, and enables HbA1c measured with high sensitivity.

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