The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 07, 2021

Filed:

Oct. 27, 2016
Applicant:

Silicon Valley Scientific, Inc., Livermore, CA (US);

Inventors:

Stevan B. Jovanovich, Livermore, CA (US);

Peter Wagner, Menlo Park, CA (US);

Assignee:

SILICON VALLEY SCIENTIFIC, INC., Livermore, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); C12N 15/1017 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01);
Abstract

A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.


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