The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 07, 2021

Filed:

Feb. 12, 2018
Applicant:

Ande Corporation, Waltham, MA (US);

Inventors:

Eugene Tan, Arlington, MA (US);

Heung Chuan Lam, Newton, MA (US);

Valery Leonidovich Bogdanov, Woburn, MA (US);

Gregory John Kellogg, Cambridge, MA (US);

John A. Wright, Billerica, MA (US);

Ulrich Hans Thomann, Stow, MA (US);

Richard F. Selden, Lincoln, MA (US);

Assignee:

ANDE CORPORATION, Waltham, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); B01L 7/00 (2006.01); G01N 21/64 (2006.01); G01N 27/447 (2006.01); B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
B01L 7/52 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502753 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); G01N 21/6402 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); G01N 21/6486 (2013.01); G01N 27/44726 (2013.01); G01N 27/44743 (2013.01); G01N 27/44782 (2013.01); G01N 27/44791 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/10 (2013.01); B01L 2200/147 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/16 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1844 (2013.01); B01L 2300/1894 (2013.01); B01L 2400/0421 (2013.01); G01N 2021/6441 (2013.01); G01N 2201/06113 (2013.01); Y10T 436/2575 (2015.01);
Abstract

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.


Find Patent Forward Citations

Loading…