The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 31, 2021

Filed:

Jun. 07, 2017
Applicant:

Terumo Bct, Inc., Lakewood, CO (US);

Inventors:

Nathan D. Frank, Arvada, CO (US);

Brian J. Nankervis, Golden, CO (US);

Dennis J. Hlavinka, Arvada, CO (US);

Thomas G. Dilorenzo, Arvada, CO (US);

Assignee:

Terumo BCT, Inc., Lakewood, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/36 (2006.01); C12M 1/34 (2006.01); C12Q 3/00 (2006.01); C12N 5/0775 (2010.01); C12M 1/12 (2006.01); B05D 7/22 (2006.01); C12M 3/04 (2006.01);
U.S. Cl.
CPC ...
C12M 23/20 (2013.01); B05D 7/22 (2013.01); C12M 23/50 (2013.01); C12M 25/10 (2013.01); C12M 25/12 (2013.01); C12M 27/10 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12M 29/18 (2013.01); C12M 41/00 (2013.01); C12M 41/48 (2013.01); C12N 5/0662 (2013.01); C12N 5/0663 (2013.01); C12Q 3/00 (2013.01); B05D 2254/00 (2013.01); B05D 2259/00 (2013.01);
Abstract

Embodiments provide for introducing a first fluid into a bioreactor and while introducing the first fluid into the bioreactor, circulating a second fluid with a reagent at a first flow rate through the bioreactor. The bioreactor may be maintained in a first orientation for a first period of time to allow at least a first portion of the reagent to coat the bioreactor. The second fluid may then be circulated at a second flow rate slower than the first flow rate to allow a second portion of the reagent to coat the bioreactor. Introduction of the first fluid may promote coating of the bioreactor by ultrafiltration. The introduction of the first fluid may be stopped. A third fluid may be circulated through the bioreactor to remove a portion of the reagent not coated on the bioreactor.


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