The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 24, 2021
Filed:
Aug. 17, 2018
Applicant:
Dana-farber Cancer Institute, Inc., Boston, MA (US);
Inventors:
David Barbie, Andover, MA (US);
Russell W. Jenkins, Boston, MA (US);
Cloud P. Paweletz, Boston, MA (US);
Elena Ivanova, Brookline, MA (US);
Amir Aref, Malden, MA (US);
Assignee:
Dana-Farber Cancer Institute, Inc., Boston, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6886 (2018.01); G01N 33/50 (2006.01); C12M 3/06 (2006.01); C12N 1/04 (2006.01); C12N 9/64 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6881 (2018.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12M 23/16 (2013.01); C12N 1/04 (2013.01); C12N 9/6491 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6881 (2013.01); G01N 33/5082 (2013.01); G01N 33/582 (2013.01); C12Y 304/24007 (2013.01);
Abstract
Provided herein are methods for evaluating tumor cell spheroids in a three-dimensional microfluidic device by determining changes in the relative levels of live cells and dead cells in aliquots cultured under different conditions. Methods described herein allow ex vivo recapitulation of the tumor microenvironment such that the in vivo effectiveness of a test compound in treating tumor tissue may be predicted.