The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Apr. 30, 2020
Applicant:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Inventors:

Clark Mason, Franklin Lakes, NJ (US);

Liping Yu, San Jose, CA (US);

Assignee:

BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); G01N 15/14 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/6886 (2018.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6886 (2013.01); G01N 15/1404 (2013.01); G01N 15/1459 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1402 (2013.01); G01N 2015/149 (2013.01);
Abstract

Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.


Find Patent Forward Citations

Loading…