The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 24, 2021
Filed:
Sep. 07, 2020
Applicant:
Northwest A&f University, Xianyang, CN;
Inventors:
Jingcheng Zhang, Xianyang, CN;
Yuemeng Huang, Xianyang, CN;
Min Zhang, Xianyang, CN;
Debao Wang, Xianyang, CN;
Yong Wang, Xianyang, CN;
Yong Zhang, Xianyang, CN;
Assignee:
NORTHWEST A&F UNIVERSITY, Xianyang, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/075 (2010.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0609 (2013.01); C12N 5/0037 (2013.01); C12N 2500/90 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01);
Abstract
A serum-free culture medium for in vitro maturation of bovine oocytes includes a Hepes-free M199 basal medium, fatty acid-free BSA, human menopausal gonadotrophin (HMG), 17β-estradiol, epidermal growth factor (EGF), L-cysteine, bFGF, Glutamax (100×), folic acid, cholic acid and CXCL12. A method of culturing bovine oocytes using such serum-free culture medium is provided.