The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Jul. 20, 2018
Applicant:

University of Macau, Macau, CN;

Inventors:

Ren-He Xu, Macau, CN;

Bin Jiang, Macau, CN;

Li Yan, Macau, CN;

Assignee:

UNIVERSITY OF MACAU, Macau, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/0775 (2010.01); A61K 9/00 (2006.01); A61K 35/28 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); A61K 9/0019 (2013.01); A61K 35/28 (2013.01); C12N 5/0662 (2013.01); C12N 5/0668 (2013.01); C12N 2500/32 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/025 (2013.01); C12N 2509/00 (2013.01); C12N 2513/00 (2013.01);
Abstract

A method of differentiating pluripotent stem cells into mesenchymal stem cells, a culture medium used in the method of differentiating pluripotent stem cells into mesenchymal stem cells and a method of performing tissue and organ regeneration by using the mesenchymal stem cells obtained by differentiation using the method of differentiating pluripotent stem cells into mesenchymal stem cells are provided. The method of differentiating pluripotent stem cells into mesenchymal stem cells comprises differentiating, completely under 3D suspension conditions, pluripotent stem cells into trophoblast-like cells using BMP4 and A8301, and then differentiating the trophoblast-like cells into mesenchymal stem cells. Neither of two differentiation processes needs passaging or replacement of a culture container.


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