The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 24, 2021

Filed:

Jul. 02, 2019
Applicant:

Zymergen Inc., Emeryville, CA (US);

Inventors:

Sean Poust, El Cerrito, CA (US);

Vytas SunSpiral, Oakland, CA (US);

William Serber, Oakland, CA (US);

Matthew Jonathan Myers, Oakland, CA (US);

Sara da Luz Areosa Cleto, Emeryville, CA (US);

Philip Weyman, Alameda, CA (US);

Assignee:

ZYMERGEN INC., Emeryville, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 15/74 (2006.01); C12R 1/01 (2006.01);
U.S. Cl.
CPC ...
C12N 1/205 (2021.05); C12N 1/20 (2013.01); C12N 15/74 (2013.01); C12R 2001/01 (2021.05);
Abstract

Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.


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