The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 17, 2021
Filed:
Oct. 27, 2016
Applicants:
The Broad Institute, Inc., Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Inventors:
Jellert Gaublomme, Cambridge, MA (US);
Aviv Regev, Cambridge, MA (US);
Assignees:
The Board Institute, Inc., Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); G01N 33/58 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/6823 (2018.01); C12Q 1/683 (2018.01);
U.S. Cl.
CPC ...
G01N 33/6845 (2013.01); C12Q 1/683 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6869 (2013.01); G01N 33/58 (2013.01); C12Q 2535/122 (2013.01); C12Q 2563/179 (2013.01); G01N 2458/10 (2013.01);
Abstract
The present invention relates to methods for high multiplex protein or cellular constituent analysis in single cells or single isolated aggregations of cellular constituents. The methods provide for embedding cells or isolated aggregations of cellular constituents in a hydrogel mesh and labeling of cellular constituents with labeling ligands linked to a nucleic acid tag. Cellular constituents can be determined using sequencing methods.