The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 17, 2021

Filed:

Jun. 22, 2018
Applicant:

Texas Tech University System, Lubbock, TX (US);

Inventors:

Dhananjay Kumar Singh, Lubbock, TX (US);

Caroline C. Ahrens, Ambler, PA (US);

Wei Li, Lubbock, TX (US);

Siva A. Vanapalli, Lubbock, TX (US);

Assignee:

Texas Tech University System, Lubbock, TX (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/02 (2006.01); G03H 1/04 (2006.01); G03H 1/08 (2006.01); G01N 33/50 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 15/0205 (2013.01); G03H 1/0443 (2013.01); G03H 1/0866 (2013.01); G01N 33/5011 (2013.01); G01N 2015/0233 (2013.01); G01N 2015/1006 (2013.01); G03H 2001/0447 (2013.01); G03H 2001/0452 (2013.01);
Abstract

The present invention includes method and device for label-free holographic screening and enumeration of tumor cells in bulk flow comprising: a laser source, a micro-objective, a pinhole device and a collimating lens, a mirror, a sample chamber with a sample flow inlet on a first side of the sample chamber and a sample flow outlet connected by a microchannel, and a detector, wherein the collimated laser beam passes through microchannel and interacts with cells in the sample to generate a respective hologram at the detector, wherein a processor calculates a numerical reconstruction from the respective hologram and generates a focused image of the numerous cells using the numerical reconstruction, wherein the numerous cells are enumerated by looking at a size, a maximum intensity and a mean intensity of the focused image.


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