The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 17, 2021

Filed:

Feb. 15, 2018
Applicant:

Commissariat a L'energie Atomique ET Aux Energies Alternatives, Paris, FR;

Inventors:

Cedric Allier, Grenoble, FR;

Melissa Baque, Grenoble, FR;

Thomas Bordy, Grenoble, FR;

Anne-Gaelle Bourdat, Nantoin, FR;

Daniel Vellou, Seyssinet, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/02 (2006.01); G01N 15/14 (2006.01); G01N 33/53 (2006.01); G01N 21/27 (2006.01); G01N 21/47 (2006.01); G01N 21/75 (2006.01); C12Q 1/6851 (2018.01); G01N 21/82 (2006.01); C12Q 1/6825 (2018.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
G01N 15/0205 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6851 (2013.01); G01N 15/1468 (2013.01); G01N 21/272 (2013.01); G01N 21/4788 (2013.01); G01N 21/75 (2013.01); G01N 21/82 (2013.01); G01N 33/5304 (2013.01); G01N 2015/0057 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/0092 (2013.01); G01N 2015/0222 (2013.01); G01N 2015/145 (2013.01); G01N 2021/755 (2013.01);
Abstract

The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (). The sample is placed between a light source () and an image sensor (). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.


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