The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

Aug. 07, 2019
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Daniel Levner, Brookline, MA (US);

Christopher David Hinojosa, Malden, MA (US);

Andries D. van der Meer, Enschede, NL;

Marinke van der Helm, Enschede, NL;

Abhishek Jain, Roslindale, MA (US);

Donald Elliot Ingber, Boston, MA (US);

Marjon Zamani, Boston, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/483 (2006.01); G01N 33/50 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); G01N 15/08 (2006.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/4833 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); G01N 15/0826 (2013.01); G01N 33/50 (2013.01); G01N 33/5005 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/082 (2013.01); B01L 2400/084 (2013.01); B01L 2400/086 (2013.01); G01N 2015/0065 (2013.01);
Abstract

Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.


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