The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

May. 13, 2020
Applicant:

China Jiliang University, Hangzhou, CN;

Inventors:

Feng Guan, Hangzhou, CN;

Jin Zhao, Hangzhou, CN;

Assignee:

CHINA JILIANG UNIVERSITY, Hangzhou, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6888 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/6888 (2013.01); C12Q 2565/537 (2013.01);
Abstract

The present invention provides a DNA barcode and method for identifying cuttlefishes, mainly depending on the diversity of mitochondrial DNA, including designing universal primers on conservative regions on both sides of the species-specific variable region, amplifying DNA from cuttlefishes, and sequencing PCR products to obtain DNA sequences, and identifying cuttlefish species based on the similarity by comparing with the species information in the database. The method is advantageous in that it can achieve efficient and specific amplification of DNA from such cephalopods as cuttlefishes, with low requirements for necessary instruments and simple operation processes. In addition, the method can be carried out in most molecular biology laboratories, which greatly improves the probability of success of molecular identification for cuttlefishes and sibling species thereof.


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