The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

Mar. 30, 2020
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

John F. Regan, San Mateo, CA (US);

Dianna Maar, Mountain House, CA (US);

Dawne N. Shelton, San Ramon, CA (US);

Tina C. Legler, Tracy, CA (US);

Samantha Cooper, Berkeley, CA (US);

Wei Yang, Dublin, CA (US);

Eli A. Hefner, Fairfield, CA (US);

Niels Klitgord, Oakland, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/6823 (2018.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6818 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/6848 (2013.01);
Abstract

System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.


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