The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

Dec. 31, 2015
Applicant:

Georgia Tech Research Corporation, Atlanta, GA (US);

Inventors:

Pamela Peralta-Yahya, Atlanta, GA (US);

Amy M. Ehrenworth, Atlanta, GA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/16 (2006.01); C12P 17/18 (2006.01); C12P 19/60 (2006.01); C12N 15/52 (2006.01); C12P 13/22 (2006.01); C12P 7/00 (2006.01); C12P 17/10 (2006.01); C12P 7/22 (2006.01); C12P 13/00 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12P 17/12 (2006.01);
U.S. Cl.
CPC ...
C12P 17/182 (2013.01); C12N 9/0006 (2013.01); C12N 9/0036 (2013.01); C12N 9/78 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12P 7/00 (2013.01); C12P 7/22 (2013.01); C12P 13/001 (2013.01); C12P 13/225 (2013.01); C12P 13/227 (2013.01); C12P 17/10 (2013.01); C12P 17/12 (2013.01); C12P 17/16 (2013.01); C12P 17/18 (2013.01); C12P 19/60 (2013.01); C12Y 101/01153 (2013.01); C12Y 106/99 (2013.01); C12Y 305/04016 (2013.01); C12Y 401/01028 (2013.01); C12Y 402/01096 (2013.01); C12Y 402/03012 (2013.01);
Abstract

Provided herein are biocatalysts and systems thereof for pterin-dependent enzymes and pathways and methods of making and using the same. Provided herein in some embodiments are biocatalysts having a pterin source and a pterin-dependent enzymatic pathway biologically coupled to the pterin source. Tetrahydrobiopterin (referred to herein as BH4 or BH 4) can be the pterin source. The BH4 can be synthesized by a tetrahydrobiopterin synthesis pathway. The tetrahydrobiopterin synthesis pathway can include a GTP cyclohydrase; a pyruvoyl tetrahydropterin synthase; a sepiapterin reductase, and/or any combination thereof. The biocatalyst can further contain a pterin-dependent enzymatic pathway. The pterin-dependent enzymatic pathway can be amino acid mono-oxygenase, phenylalanine hydroxylase, tryptophan hydroxylase, tyrosine hydroxylase, nitric oxide synthase, alkylglycerol monooxygenase, and/or any combination thereof.


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