The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 10, 2021

Filed:

Nov. 16, 2016
Applicant:

Kenichi Yamahara, Kyoto, JP;

Inventors:

Kenichi Yamahara, Kyoto, JP;

Shigeo Sudo, Fukuyama, JP;

Toshita Sudo, Fukuyama, JP;

Tomoharu Hiroshima, Fukuyama, JP;

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/16 (2015.01); A61K 35/19 (2015.01); A61K 35/17 (2015.01); C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0662 (2013.01); A61K 35/16 (2013.01); A61K 35/17 (2013.01); A61K 35/19 (2013.01); C12N 2500/00 (2013.01); C12N 2501/115 (2013.01); C12N 2501/135 (2013.01); C12N 2501/15 (2013.01); C12N 2501/165 (2013.01); C12N 2501/20 (2013.01); C12N 2501/21 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/231 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2305 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/2308 (2013.01); C12N 2501/2309 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/2313 (2013.01); C12N 2501/24 (2013.01); C12N 2501/25 (2013.01);
Abstract

Provided is a method for producing an economical bovine serum composition containing many factors useful for cell proliferation. The method includes a step of performing an anticoagulation treatment of bovine whole blood with an anticoagulant, a step of obtaining a buffy coat and a fraction with a heavier specific gravity than that of the buffy coat from the anticoagulated whole blood, and a step of promoting and activating an interaction between the obtained leukocytes and platelets at a given temperature for not less than a given time to cause secretion or release of a humoral factor from the leukocytes and/or platelets and performing a recoagulation treatment of blood components including the humoral factor with a re-coagulating agent.


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