The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 03, 2021

Filed:

Oct. 24, 2014
Applicant:

Kikkoman Corporation, Noda, JP;

Inventors:

Yosuke Masakari, Noda, JP;

Atsushi Ichiyanagi, Noda, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/96 (2006.01); C12Q 1/26 (2006.01); C12N 9/06 (2006.01); G01N 33/72 (2006.01); C12Q 1/28 (2006.01); G01N 21/78 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/26 (2013.01); C12N 9/0032 (2013.01); C12Q 1/28 (2013.01); C12Y 105/03 (2013.01); G01N 21/78 (2013.01); G01N 33/725 (2013.01); C12Y 111/01 (2013.01); G01N 2333/805 (2013.01); G01N 2333/908 (2013.01); G01N 2333/90672 (2013.01);
Abstract

This invention provides an amadoriase that acts on the β-chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbA1c using such amadoriase. The method for measurement of HbA1c involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to αF6P and oxidizes the HbA1c β-chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbA1c enable quantification of HbA1c to be performed rapidly, simply, and accurately.


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