The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 03, 2021
Filed:
Jun. 21, 2017
Applicant:
Nanyang Technological University, Singapore, SG;
Inventors:
Harshyaa Makhija, Singapore, SG;
Peter Droge, Singapore, SG;
Assignee:
Nanyang Technological University, Singapore, SG;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 15/90 (2006.01); C07K 14/705 (2006.01); C07K 16/00 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C07K 14/7051 (2013.01); C07K 14/70578 (2013.01); C07K 16/00 (2013.01); C07K 16/2803 (2013.01); C12N 15/907 (2013.01); C07K 2317/622 (2013.01); C07K 2319/00 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C12N 2800/107 (2013.01); C12N 2800/30 (2013.01);
Abstract
The present invention provides a method of stably integrating a DNA sequence of interest into a target genomic DNA sequence of a host cell, wherein the target genomic DNA sequence comprises a nucleotide sequence that is at least 80% homologous to the sequence as set forth in SEQ ID NO:1, as well as a kit for use in said method. Also provided is a method of generating a circular DNA construct essentially consisting of a DNA sequence of interest and a nucleotide sequence that is at least 80% homologous to the sequence as set forth in SEQ ID NO:3.