The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 03, 2021
Filed:
Aug. 28, 2014
Applicant:
Amgen Inc., Thousand Oaks, CA (US);
Inventors:
Huiren Zhao, Thousand Oaks, CA (US);
Grace Ki Jeong Lee, Simi Valley, CA (US);
Thomas Wolfe, Simi Valley, CA (US);
Cherylene Plewa, Simi Valley, CA (US);
Jackie Z. Sheng, Thousand Oaks, CA (US);
Assignee:
Amgen Inc., Thousand Oaks, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C12N 15/86 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01); C12N 2750/14152 (2013.01);
Abstract
The present invention is directed to an in vitro method of producing a recombinant AAV virion in a mammalian host cell that comprises a functional adenoviral E1A gene and rAAV virions made by the method. Host cells are transfected with varying ratios of plasmids comprising the E1A gene and virions.