The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 27, 2021
Filed:
Aug. 22, 2017
Applicant:
Zymo Research Corporation, Irvine, CA (US);
Inventors:
Xiyu Jia, Newport Beach, CA (US);
Onyinyechi Chimaokereke, Tustin, CA (US);
Lam Nguyen, Fountain Valley, CA (US);
Assignee:
ZYMO RESEARCH CORPORATION, Irvine, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01); C12Q 1/6827 (2013.01); C12Q 2545/114 (2013.01); C12Q 2521/331 (2013.01);
Abstract
In a first aspect, the invention concerns a method for detecting or quantifying DNA methylation at a locus. In one embodiment, a methylation-sensitive endonuclease is formulated together with a polymerase enzyme in an appropriate reaction mixture such that amplification of DNA occurs in a methylation specific manor. Quantitative DNA amplification at selected loci can be used to determine the level of methylation. Kits and reagents for performing such methods are also provided.