The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 20, 2021
Filed:
Oct. 31, 2019
Applicant:
Zymergen Inc., Emeryville, CA (US);
Inventors:
Erik Jedediah Dean, Lafayette, CA (US);
Kedar Patel, Fremont, CA (US);
Aaron Miller, Berkeley, CA (US);
Kunal Mehta, Oakland, CA (US);
Philip Weyman, Alameda, CA (US);
Assignee:
Zymergen Inc., Emeryville, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C40B 50/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1068 (2013.01); C12N 15/1093 (2013.01); C40B 50/06 (2013.01);
Abstract
The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.