The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 13, 2021
Filed:
Jun. 11, 2015
Applicant:
Illumina Cambridge Limited, Nr Saffron Walden, GB;
Inventors:
Isabelle Marie Julia Rasolonjatovo, Essex, GB;
Jonathan Mark Boutell, Cambridge, GB;
Vincent Peter Smith, Cambridge, GB;
Roberto Rigatti, Cambridge, GB;
Assignee:
Illumina Cambridge Limited, Essex, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6874 (2013.01);
Abstract
The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.