The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 13, 2021

Filed:

Sep. 15, 2017
Applicant:

Klawego Gmbh & Co. KG, Heddesheim, DE;

Inventors:

Klaus Gottschall, Heddesheim, DE;

Lothar Britsch, Reute, DE;

Evelyn Gottschall, Heddesheim, DE;

Assignee:

Klawego GmbH & Co. KG, Heddesheim, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 15/34 (2006.01); B01J 20/26 (2006.01); B01J 20/28 (2006.01); B01J 20/285 (2006.01); B01J 20/30 (2006.01); C07K 16/42 (2006.01); C07K 1/34 (2006.01); C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/22 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C07K 1/165 (2013.01); B01D 15/34 (2013.01); B01J 20/267 (2013.01); B01J 20/285 (2013.01); B01J 20/28042 (2013.01); B01J 20/28088 (2013.01); B01J 20/28097 (2013.01); B01J 20/3085 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/34 (2013.01); C07K 16/00 (2013.01); C07K 16/42 (2013.01); B01J 20/28083 (2013.01); B01J 20/28085 (2013.01);
Abstract

Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rand the impurity compound being characterized by a hydrodynamic radius R, wherein R>R, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that R<Rand R>R; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.


Find Patent Forward Citations

Loading…