The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 29, 2021

Filed:

Aug. 14, 2017
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Emily D. Crawford, San Francisco, CA (US);

Eric D. Chow, Richmond, CA (US);

Joseph L. DeRisi, San Francisco, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 9/22 (2013.01); C12N 15/10 (2013.01); C12N 15/11 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01); C12N 2310/20 (2017.05);
Abstract

A method of sample analysis is provided. In some embodiments, the method comprises: (a) digesting a mixed nucleic acid sample with a plurality of reprogrammed nucleic acid-directed endonucleases that target sequences of interest to produce a digested sample, wherein at least some of the fragments in the digested sample comprise: (i) a sequence of interest and (ii) at least one ligatable end that has been generated by endonuclease cleavage; (b) enriching for fragments that contain the sequence of interest; and (c) analyzing the enriched fragments. Kits for performing the method are also provided.


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