The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 29, 2021

Filed:

Oct. 07, 2014
Applicant:

Prestige Biopharma Pte. Ltd., Singapore, SG;

Inventors:

Soo Kwang Kim, Daejeon, KR;

Young Min Kim, Gyeonggi-do, KR;

Yong Gyu Son, Gyeongsangnam-do, KR;

Yong Ho Ahn, Daejeon, KR;

Dong Heon Lee, Daejeon, KR;

Yang Soon Lee, Daejeon, KR;

Hee Jung Jun, Daejeon, KR;

Yu Bin Choi, Daejeon, KR;

Eun A Kim, Incheon, KR;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C07K 16/24 (2006.01); C07K 16/00 (2006.01); C07K 1/14 (2006.01); C07K 16/18 (2006.01); C07K 16/22 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C07K 1/14 (2013.01); C07K 16/00 (2013.01); C07K 16/18 (2013.01); C07K 16/22 (2013.01); C07K 16/24 (2013.01); C07K 16/241 (2013.01); C07K 16/244 (2013.01); C07K 16/2818 (2013.01); C07K 16/2866 (2013.01); C12N 2510/02 (2013.01); C12N 2840/203 (2013.01);
Abstract

The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including 'promoter-UTR-intron-antibody light chain gene-polyA' and a second expression cassette including 'promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA'. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.


Find Patent Forward Citations

Loading…