The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 29, 2021

Filed:

Nov. 24, 2016
Applicants:

Kyushu University, National University Corporation, Fukuoka, JP;

Takara Bio Inc., Kusatsu, JP;

Inventors:

Yoshizumi Ishino, Fukuoka, JP;

Sonoko Ishino, Fukuoka, JP;

Takeshi Yamagami, Fukuoka, JP;

Takashi Uemori, Otsu, JP;

Nariaki Takatsu, Kusatsu, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12N 1/20 (2006.01); C12N 15/52 (2006.01); C12N 15/63 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1252 (2013.01); C12N 1/20 (2013.01); C12N 15/52 (2013.01); C12N 15/63 (2013.01); C12N 15/70 (2013.01); C12N 15/74 (2013.01); C12P 19/34 (2013.01);
Abstract

The present invention relates to a(Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.


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