The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 29, 2021
Filed:
Mar. 25, 2016
Applicant:
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Inventors:
Assignee:
Chugai Seiyaku Kabushiki Kaisha, Tokyo, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/28 (2006.01); A61K 38/00 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2866 (2013.01); A61K 38/00 (2013.01); C12N 5/0018 (2013.01); C12N 5/0682 (2013.01); C12P 21/00 (2013.01); C07K 2317/14 (2013.01); C07K 2317/24 (2013.01); C12N 2500/20 (2013.01); C12N 2500/80 (2013.01); C12N 2510/02 (2013.01);
Abstract
The present invention provides a method of cell culture comprising adding a cell-containing seed medium to an initial medium and starting to culture the cell in the initial medium, wherein the initial medium has an organism-derived culture medium additive added thereto and the amount of the C-terminal amidated species in the produced protein is controlled by the copper content of the initial medium at the start of cell culture.