The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 22, 2021

Filed:

Oct. 20, 2019
Applicant:

National Institute of Fisheries Science, Busan, KR;

Inventors:

Miyoung Cho, Busan, KR;

Myoung Ae Park, Busan, KR;

Bo-Young Jee, Busan, KR;

Seong Don Hwang, Busan, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6897 (2018.01); C12Q 1/70 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6837 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6897 (2013.01); C12Q 1/705 (2013.01); C12Q 2525/107 (2013.01);
Abstract

Genetic markers are described for discriminating or detecting viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same is disclosed, in which the method includes selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish are infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.


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