The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 15, 2021
Filed:
Jul. 08, 2019
Applicant:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Inventors:
Aruna Ayer, Santa Clara, CA (US);
Preethi Sarvabhowman, Santa Clara, CA (US);
Charles Schwab, San Ramon, CA (US);
Assignee:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); C12N 9/96 (2006.01); C12Q 1/6806 (2018.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 9/1252 (2013.01); C12N 9/96 (2013.01); C12Q 1/6806 (2013.01); C12Y 207/07007 (2013.01);
Abstract
The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.