The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 15, 2021

Filed:

Jun. 30, 2017
Applicant:

Kuehnle Agrosystems, Inc., Honolulu, HI (US);

Inventors:

Robert J. Schurr, Honolulu, HI (US);

Adelheid R. Kuehnle, Honolulu, HI (US);

Assignee:

Kuehnle AgroSystems, Inc., Honolulu, HI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/12 (2006.01); C12N 15/82 (2006.01); C12P 23/00 (2006.01); A23K 50/80 (2016.01); A23K 20/10 (2016.01); A01N 65/03 (2009.01); C12P 3/00 (2006.01); C12P 5/02 (2006.01); C12P 7/26 (2006.01); C12P 9/00 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 15/82 (2013.01); A01N 65/03 (2013.01); A23K 20/10 (2016.05); A23K 50/80 (2016.05); C12N 1/12 (2013.01); C12P 3/00 (2013.01); C12P 5/026 (2013.01); C12P 7/26 (2013.01); C12P 9/00 (2013.01); C12P 23/00 (2013.01); G01N 33/5097 (2013.01); G01N 2333/405 (2013.01); Y02E 50/30 (2013.01);
Abstract

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response.


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