The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 01, 2021
Filed:
Jun. 02, 2015
Eth Zurich, Zurich, CH;
Yaakov Benenson, Basel, CH;
Benjamin Hafliger, Aarau, CH;
ETH ZURICH, Zurich, CH;
Abstract
The present invention relates to a method for identifying miRNA (microRNA)-modulating compounds by contacting a compound of interest with a cell comprising (i) a 'miRNA-specific module' that reports specific miRNA target sequence binding by means of a first reporter product; (ii) a 'non-specific RNAi module' that reports altered expression of one or more endogenous miRNAs with baseline expression levels in the cell (“non-specific miRNA markers”) by means of a second reporter product; and optionally (iii) a “gene expression module” that reports global changes in gene expression; and subsequent detection of changes in reporter product expression. The advantage of this method is that non-specific miRNA- and/or RNAi-effects of the compound of interest are detected and false positive results due to the structural similarity of different miRNAs and the commonly shared maturation pathway for most miRNAs are substantially reduced over conventional miRNA screening assays.