The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 01, 2021
Filed:
Mar. 28, 2017
Applicant:
Boreal Genomics, Inc., Vancouver, CA;
Inventors:
Andrea Marziali, North Vancouver, CA;
Joel Pel, Vancouver, CA;
Assignee:
Boreal Genomics, Inc., Vancouver, CA;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6855 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); C40B 20/04 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6853 (2018.01); C40B 40/08 (2006.01); C12Q 1/6876 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6855 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C40B 20/04 (2013.01); C40B 40/08 (2013.01); C12Q 1/6853 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01);
Abstract
The invention generally relates to sequencing library preparation methods. In certain embodiments, two or more template nucleic acids are joined together by a linking molecule, such as a PEG derivative. Identical copies of a nucleic acid fragment or both strands of a duplex fragment may be linked together. The linked nucleic acids are amplified, creating linked amplicons. Emulsion PCR with linked primers creates linked template nucleic acids for seeding sequencing clusters and errors can be readily identified by their presence on only one of the linked fragments.