The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 01, 2021
Filed:
Apr. 13, 2018
Applicant:
Life Technologies Corporation, Carlsbad, CA (US);
Inventors:
Dumitru Brinza, Montara, CA (US);
Fiona Hyland, San Mateo, CA (US);
Assignee:
Life Technologies Corporation, Carlsbad, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6806 (2018.01); G16B 30/00 (2019.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6869 (2013.01); G16B 30/00 (2019.02);
Abstract
Systems and method for validation of sequencing results can amplify a target region of a nucleic acid sample in the presence of a primer pool including target specific and variant specific primers. The variant specific primers can include variant specific barcodes and variant specific sequences. An amplicon can be sequenced to determine the sequence of the variant specific barcode. The variant can be identified based on the sequence of the variant specific barcode, and the location of the variant can be determined by mapping the amplicon to a reference sequence.