The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 25, 2021
Filed:
Mar. 03, 2016
Postech Academy-industry Foundation, Pohang-si, KR;
The Regents of the University of Michigan, Ann Arbor, MI (US);
Jae Sung Park, Pohang-si, KR;
Joseph M Labuz, Ann Arbor, MI (US);
Shuichi Takayama, Ann Arbor, MI (US);
Hyun Woo Shin, Busan, KR;
Chung Min Han, Pohang-si, KR;
POSTECH ACADEMY-INDUSTRY FOUNDATION, Pohang-si, KR;
THE REGENTS OF THE UNIVERSITY OF MICHIGEN, Ann Arbor, MI (US);
Abstract
Disclosed is a method of isolating extracellular vesicles using an aqueous two-phase system (ATPS), including (a) preparing an ATPS by mixing a first material and a second material, which are immiscible with each other, with a body fluid or an aqueous solution containing extracellular vesicles and (b) isolating extracellular vesicles concentrated in the second material of the ATPS. This method can exhibit very high isolation efficiency, a simple isolation manner, and a very short isolation time. The isolation of extracellular vesicles using the ATPS requires no ultracentrifuge and achieves almost 100% isolation efficiency within a short time of about 10˜20 min, and thus the method of the invention is practical, is economical due to low costs thereof, can increase the purity of extracellular vesicles contaminated with protein, enables the diagnosis of disease using the isolated extracellular vesicles, and can be applied to various fields using extracellular vesicles.