The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 25, 2021

Filed:

Sep. 06, 2018
Applicant:

The Charles Stark Draper Laboratory, Inc., Cambridge, MA (US);

Inventors:

Jeffrey T. Borenstein, Newton, MA (US);

Jenna L. Balestrini, Boston, MA (US);

Vishal Tandon, Roxbury Crossing, MA (US);

Louis B. Kratchman, Quincy, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12M 3/00 (2006.01); C12M 1/42 (2006.01); C12N 15/87 (2006.01); C12N 13/00 (2006.01); C12M 1/12 (2006.01); C12M 3/06 (2006.01);
U.S. Cl.
CPC ...
C12M 35/02 (2013.01); B01L 3/502753 (2013.01); C12M 23/16 (2013.01); C12M 25/02 (2013.01); C12N 13/00 (2013.01); C12N 15/87 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0887 (2013.01);
Abstract

Microfluidic devices and associated methods are disclosed. A microfluidic device includes a target entrainment channel and an effluent channel on opposing sides of a semipermeable membrane. A restrictor channel that is narrower than the effluent channel is interposed between the semipermeable membrane and the effluent channel. Fluid that flows from the target entrainment channel, through the semipermeable membrane and the restrictor channel to the effluent channel, pins target cells along the center of the target entrainment channel for electroporation using an electrode in the channel.


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