The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 25, 2021
Filed:
Mar. 28, 2017
Applicant:
Ultragenyx Pharmaceutical Inc., Novato, CA (US);
Inventors:
Christopher J. Morrison, Arlington, MA (US);
James D. Maratt, Belmont, MA (US);
Assignee:
Ultragenyx Pharmaceutical Inc., Novato, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/761 (2015.01); C12N 15/85 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
A61K 35/761 (2013.01); C12N 7/00 (2013.01); C12N 15/85 (2013.01); C12N 15/86 (2013.01); C12N 2523/00 (2013.01); C12N 2710/10361 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01);
Abstract
The present disclosure generally relates to methods of protecting the genomic integrity and/or biological activity of AAV viral particles in a sample containing both AAV particles and helper virus particles during heat inactivation. The methods include heating, to a temperature greater than or equal to 45° C., a sample containing helper virus particles, AAV particles, and a buffer. The buffer includes a concentration of 10 mM or greater kosmotropic salts and/or a concentration of 10 mM or greater of divalent or trivalent cations.