The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 18, 2021
Filed:
Apr. 11, 2018
Calico Life Sciences Llc, South San Francisco, CA (US);
Andrew Gregory York, San Francisco, CA (US);
Sanjay Ramesh Varma, Oakland, CA (US);
CALICO LIFE SCIENCES LLC, South San Francisco, CA (US);
Abstract
Fluorescence imaging based on stimulated emission. In one example a system includes an excitation source () that excites fluorescent markers () in a sample () with an excitation beam (), a stimulation source () that stimulates the excited fluorescent markers () to produce stimulated emission (), and an objective lens () that receives the stimulation beam () and the stimulated emission (). The system further includes a phase shifting and attenuation apparatus () configured to receive the stimulation beam () and the stimulated emission () from the objective lens (), to attenuate the stimulation beam (), and to induce a relative phase shift between a reference beam (optionally being at least a portion of the stimulation beam) and the stimulated emission, thereby causing constructive interference between the reference beam and the stimulated emission. The system further includes an optical apparatus () configured to focus the stimulated emission onto an image plane, and a detector () positioned at the image plane and configured to image the stimulated emission.