The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 18, 2021
Filed:
Jul. 29, 2019
Applicant:
Jiangnan University, Wuxi, CN;
Inventors:
Fang Fang, Wuxi, CN;
Tao Yang, Wuxi, CN;
Jingwen Zhou, Wuxi, CN;
Jian Chen, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jie Xu, Wuxi, CN;
Assignee:
JIANGNAN UNIVERSITY, Wuxi, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12N 15/52 (2006.01); A23L 5/20 (2016.01);
U.S. Cl.
CPC ...
C12N 9/0004 (2013.01); A23L 5/25 (2016.08); C12N 15/52 (2013.01);
Abstract
The present disclosure provides a multicopper oxidase mutant with improved salt tolerance. Threonine at site 317 of wild-type multicopper oxidase WT was mutated to asparagine, leucine at site 386 was mutated to tyrosine, and serine at site 427 was mutated to glutamic acid by site-directed mutagenesis to obtain a mutant T317N-L386Y-S427E. Compared with WT, the tolerance of T317N-L386Y-S427E to 6%, 9%, 12%, 15% and 18% NaCl (W/V) is improved.