The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 11, 2021
Filed:
Feb. 18, 2016
Applicant:
Geneplus—beijing, Beijing, CN;
Inventors:
Xiaoxing Lv, Beijing, CN;
Xin Yi, Beijing, CN;
Meiru Zhao, Beijing, CN;
Yanfang Guan, Beijing, CN;
Tao Liu, Beijing, CN;
Ling Yang, Beijing, CN;
Assignee:
Geneplus—Beijing, Beijing, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/10 (2006.01); G16B 30/00 (2019.01); G16B 40/00 (2019.01); G16B 40/30 (2019.01); G16B 20/20 (2019.01); C12Q 1/6806 (2018.01); A61P 35/00 (2006.01); C12Q 1/6853 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6886 (2018.01); C40B 50/10 (2006.01); G16B 30/10 (2019.01); G16H 20/10 (2018.01); C40B 40/08 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1072 (2013.01); A61P 35/00 (2018.01); C12N 15/1058 (2013.01); C12N 15/1065 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6886 (2013.01); C40B 50/10 (2013.01); G16B 20/20 (2019.02); G16B 30/00 (2019.02); G16B 40/00 (2019.02); G16B 40/30 (2019.02); C12N 15/1003 (2013.01); C12N 15/1093 (2013.01); C40B 40/08 (2013.01); G16B 30/10 (2019.02); G16H 20/10 (2018.01);
Abstract
The present invention provides a low-frequency mutation enrichment sequencing method for free target DNA in plasma, comprising plasma DNA extraction and library construction, general library TT COLD PCR amplification enrichment, probe enrichment capture, PCR and sequencing of captured products, and positive and negatice double-strand error-correction low-frequency information analysis.