The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 11, 2021
Filed:
Apr. 04, 2018
Applicant:
Georgetown University, Washington, DC (US);
Inventors:
Richard Schlegel, Rockville, MD (US);
Xuefeng Liu, Gaithersburg, MD (US);
Assignee:
Georgetown University, Washington, DC (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12Q 1/6883 (2018.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0683 (2013.01); C12N 5/067 (2013.01); C12N 5/0625 (2013.01); C12N 5/0631 (2013.01); C12N 5/0688 (2013.01); C12Q 1/6883 (2013.01); G01N 33/5091 (2013.01); C12N 2500/05 (2013.01); C12N 2500/40 (2013.01); C12N 2501/01 (2013.01); C12N 2501/11 (2013.01); C12N 2501/33 (2013.01); C12N 2501/39 (2013.01); C12N 2501/727 (2013.01); C12N 2502/13 (2013.01); C12N 2502/1323 (2013.01); C12N 2503/02 (2013.01); C12N 2510/04 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/158 (2013.01);
Abstract
The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.