The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 27, 2021

Filed:

Sep. 11, 2015
Applicants:

Board of Regents of the University of Texas System, Austin, TX (US);

Amprion, Inc., Houston, TX (US);

Inventors:

Claudio Soto Jara, Friendswood, TX (US);

Mohammad Shahnawaz, Houston, TX (US);

Russell M. Lebovitz, Oakland, CA (US);

Benedikt K. Vollrath, San Diego, CA (US);

Assignees:

Amprion, Inc., San Francisco, CA (US);

The Board of Regents of the University of Texas System, Austin, TX (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6896 (2013.01); G01N 2333/4703 (2013.01); G01N 2800/2835 (2013.01); G01N 2800/52 (2013.01);
Abstract

Methods and kits are provided for amplifying and detecting αS proteins from samples, for example, from patients having Parkinson's Disease. For example, a method for determining a presence of a soluble, misfolded αS protein may include: contacting the sample with a monomeric, folded αS protein to form an incubation mixture; conducting an incubation cycle two or more times on the incubation mixture effective to form an amplified portion of misfolded αS protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded αS protein in the presence of the soluble, misfolded αS protein; physically disrupting the incubation mixture effective to at least partly de-aggregate at least a portion of a misfolded αS aggregate present; and determining the presence of the soluble, misfolded αS protein in the sample by detecting at least a portion of the soluble, misfolded αS protein.


Find Patent Forward Citations

Loading…