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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10988733 B1

PDFFull Text
Date of Patent:
Apr. 27, 2021

Filed:

May. 19, 2020

Methods for obtaining pluripotent adult olfactory stem cells from an olfactory mucosa tissue

Applicant:

China Medical University, Taichung, TW;

Inventors:

Woei-Cherng Shyu, Taichung, TW;

Chung-Y. Hsu, Taichung, TW;

Der-Yang Cho, Taichung, TW;

Chen-Huan Lin, Taichung, TW;

Wei Lee, Taipei, TW;

San-Yuan Chen, Hsinchu, TW;

Long-Bin Jeng, Taichung, TW;

Chang-Hai Tsai, Taichung, TW;

Assignee:

China Medical University, Taichung, TW;

Attorney:
Primary Examiner:

Chang-Yu Wang

Int. Cl.
CPC ...
C12N 5/071 (2010.01); C12N 5/074 (2010.01); C12N 5/0797 (2010.01); C12N 5/0793 (2010.01); C12N 5/079 (2010.01); C12N 5/00 (2006.01); C12N 5/0789 (2010.01); A61K 48/00 (2006.01); A61K 35/30 (2015.01); A61K 35/545 (2015.01); A61K 39/00 (2006.01); G01N 33/50 (2006.01); G01N 33/483 (2006.01); G01N 33/48 (2006.01); C12N 5/095 (2010.01); C12N 5/07 (2010.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0607 (2013.01); A61K 35/30 (2013.01); A61K 35/545 (2013.01); C12N 5/062 (2013.01); C12N 5/0623 (2013.01); A01K 2267/03 (2013.01); A01K 2267/0318 (2013.01); A61K 35/12 (2013.01); A61K 2039/6006 (2013.01); C12N 5/00 (2013.01); C12N 5/06 (2013.01); C12N 5/0602 (2013.01); C12N 5/0618 (2013.01); C12N 5/0619 (2013.01); C12N 5/0625 (2013.01); C12N 5/0632 (2013.01); C12N 5/0688 (2013.01); C12N 5/0695 (2013.01); C12N 5/0696 (2013.01); C12N 2502/00 (2013.01); C12N 2502/03 (2013.01); C12N 2502/08 (2013.01); C12N 2502/083 (2013.01); C12N 2502/088 (2013.01); C12N 2502/09 (2013.01); C12N 2502/27 (2013.01); C12N 2502/45 (2013.01); C12N 2506/03 (2013.01); C12N 2506/08 (2013.01); C12N 2506/09 (2013.01); C12N 2506/27 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01); C12N 2533/00 (2013.01); C12N 2535/00 (2013.01); C12N 2537/00 (2013.01); C12N 2539/00 (2013.01); G01N 33/48 (2013.01); G01N 33/483 (2013.01); G01N 33/50 (2013.01); G01N 33/5005 (2013.01); G01N 33/5008 (2013.01); G01N 33/5023 (2013.01); G01N 33/5044 (2013.01); G01N 33/5058 (2013.01); G01N 33/5073 (2013.01);
Abstract

A method for obtaining a plurality of pluripotent adult olfactory stem cells (APOSCs) includes isolating the APOSCs, culturing the isolated APOSCs in a sphere culture medium, and collecting the cultured APOSCs that express Bmi-1 (B-lymphoma moloney murine leukemia virus insertion region-1), Oct-4 (Octamer-binding transcription factor 4), Sox-2 (Sex-determining region Y (SRY)-box 2), Nanog, SSEA-4 (Stage-specific embryonic antigen-4), ki67, c-Myc, KLF-4 (Kruppel Like Factor 4), K14 (Cytokeratin 14) and ICAM-1 (Intercellular Adhesion Molecule 1).

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